Gerard Pique popped up with a crucial winner when it mattered as the Barcelona defender saved his side’s blushes with an 87th-minute goal.
Spain struggled to find a way past a resolute Czech Republic defence and Petr Cech until three minutes to go.
The holders move level on points with Croatia, who beat Turkey on Sunday.
Barcelona defender Gerard Pique saved the day for the reigning champions by scoring with just three minutes left on the clock
Pique leaps in the air to nod the ball past a helpless Petr Cech and seal the three points for his country in Toulouse
Sergio Ramos chases fellow Spain defender Pique after the centre back headed home from inside the penalty area to win the match
Spain striker Alvaro Morata squandered several chances during his side’s opening game of the tournament in France
Morata’s fails to score from close range as his effort heads straight towards the hands of the experienced former Chelsea keeper
Chelsea midfielder Cesc Fabregas puts his hands to his mouth after squandering a chance to put his side in the lead
Spain captain Ramos shows his frustration after failing to find a way past Cech in the Czech Republic goal
Cech sticks out his right leg to save an effort from Ramos while his Czech Republic team-mates look on in hope of keeping the score at 0-0
Barcelona ace Andres Iniesta and former Arsenal midfielder Tomas Rosicky do battle for the ball during a midfield tussle
Spain left back Jordi Alba is tackled by Czech Republic’s Pavel Kaderabek who puts in a committed challenge to stop the Barcelona man
Morata wipes out an assistant referee after stretching for the ball before running into the Polish official on the sideline
Czech Republic defender Tomas Sivok reacts after going close to breaking the deadlock during the match at the Stadium Municipal
David de Gea shows his athleticism to dive down to his left-hand side and keep the ball out of his goal
Ramos questions a decision made by Polish referee Szymon Marciniak (left) as his Spain team-mate Fabregas watches on
Fabregas (left) makes way for Thiago Alcantara (right) as Spain national team boss Vicente del Bosque attempts to win the fixture
Manchester United goalkeeper De Gea was given the nod to start ahead of former Spain No 1 Iker Casillas
Pique (left) and Tomas Necid (right) keep their eyes fixed on the ball as they prepare to win possession for their side
De Gea shakes the hand of Arsenal and Czech Republic custodian Cech ahead of the Euro 2016 Group D clash in Toulouse