The striker was sent off for two bookable offences in the draw against West Ham and will not feature against Manchester United.
The Football Association have confirmed Jamie Vardy’s additional one-game ban, meaning he will miss Leicester’s trip to Manchester United on Sunday.
Leicester will win the title with victory at Old Trafford but they will be without their star striker as they bid to complete their fairytale triumph.
The England striker was sent off against West Ham earlier this month after receiving a second yellow card for an alleged dive.
However, it was his actions after the red card, where he gesticulated towards referee Jon Moss, which he has received further sanctions for.
Vardy has already served the suspension for the red card, sitting out of Leicester’s rout over Swansea on Sunday.
An FA statement read: “Following an Independent Regulatory Commission hearing, Jamie Vardy has been given a one-match suspension with immediate effect, fined £10,000 and warned as to his future conduct.
“The Leicester City forward had accepted an FA charge of improper conduct following his second half dismissal against West Ham United on Sunday 17 April 2016.
“Meanwhile, the club has been fined £20,000 after accepting an FA charge of failing to ensure its players conducted themselves in an orderly fashion.
“The standard penalty, which the club accepted, followed an 83rd minute incident during the same fixture.
Despite being dismissed against West Ham, Vardy has been backed by several figures including England boss Roy Hodgson.
Following the red card, Vardy pointed a finger at referee Jonathan Moss and swore at the official.
The panel today ruled his aggression warranted a further one-match ban.
Vardy collected a special award at the PFA Awards after scoring in 11 successive Premier League games earlier this season.
Speaking at the ceremony, he said: “I’m over the moon but I wouldn’t have been able to do it without the team.
“Its like a fairground ride and we are just enjoying ourselves and we will keep enjoying ourselves for the last few games. There are no nerves at all.”